![]() Get Access UCSD Researchers can get access to Prism software. You can organize your data effectively, perform the right analysis, get actionable help as you go, graph, and share your work. ![]() Our results highlight a distinct chemical reprogramming pathway that leads to a shortcut for the generation of human pluripotent stem cells, which represents a powerful strategy for human cell fate manipulation. Prism is the preferred analysis and graphing solution purpose-built for scientific research. We found that this optimized protocol enabled a more direct reprogramming process by promoting cell proliferation and oxidative phosphorylation metabolic activities at the early stage. Here, by screening for small molecule boosters and systematically optimizing the original condition, we have established a robust, chemically defined reprogramming protocol, which greatly shortens the induction time from ∼50 days to a minimum of 16 days and enables highly reproducible and efficient generation of hCiPSCs from all 17 tested donors. P2P group has released the updated version of GraphPad Prism. However, the utility of this chemical approach is currently hampered by slow kinetics. This article has been published at - visit our site for full content. We recently demonstrated the chemical reprogramming of human somatic cells to pluripotent stem cells (hCiPSCs), which provides a robust approach for cell fate manipulation.
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